The leader sequence of the Escherichia coli lysC gene is involved in the regulation of LysC synthesis.
نویسندگان
چکیده
In Escherichia coli and Bacillus subtilis, long leader sequences are found upstream of the lysC coding sequences which encode lysine-sensitive aspartokinase. Highly conserved regions exist between these sequences. Mutations leading to constitutive expression of the E. coli lysC gene have been localised within these conserved regions, indicating that they participate in the lysine-mediated repression mechanism of lysC expression.
منابع مشابه
Nucleotide sequence of the promoter region of the E. coli lysC gene.
The regulatory region of the lysC gene (that encodes the lysine-sensitive aspartokinase of Escherichia coli) has been identified and purified by the use of lysC-lacZ fusions. Its regulatory sequence has been determined. No signals similar to those described in the case of an attenuation mechanism could be found in the long leader sequence existing between the starts of transcription and of tran...
متن کاملThe L box regulon: lysine sensing by leader RNAs of bacterial lysine biosynthesis genes.
Expression of amino acid biosynthesis genes in bacteria is often repressed when abundant supplies of the cognate amino acid are available. Repression of the Bacillus subtilis lysC gene by lysine was previously shown to occur at the level of premature termination of transcription. In this study we show that lysine directly promotes transcription termination during in vitro transcription with B. ...
متن کاملRegulation of aspartokinase III synthesis in Escherichia coli: isolation of mutants containing lysC-lac fusions.
Mutants containing fusions of the lac gene to the lysC gene were isolated. In these, the expression of beta-galactosidase was regulated by lysine (and arginine), as previously described for aspartokinase III.
متن کاملGlobal gene expression profiling of wild type and lysC knockout Escherichia coli W3110.
Aspartokinase III, encoded by lysC, is responsible for the first step of lysine biosynthesis in Escherichia coli. In this study, a lysC knockout E. coli W3110 strain was generated to study the differential gene expression profiles of wild type and lysC knockout strains. Several significant changes were observed, including biosynthesis of lysine, oxaloacetate, alpha-ketoglutarate and glutamate g...
متن کاملP2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC.
Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase beta' subunit and is nonpermissive for plating of bacteriophage P2. P2 gor mutants productively infect 397c and define a new gene, lysC, encoded by a reading frame that extensively overlaps the P2 lysis accessory gene, lysB. The unusual location of lysC with...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- FEMS microbiology letters
دوره 169 1 شماره
صفحات -
تاریخ انتشار 1998